Separation of manganese peroxidase isoenzymes on strong anion-exchange monolithic column using pH-salt gradient.
Identifieur interne : 000864 ( Main/Exploration ); précédent : 000863; suivant : 000865Separation of manganese peroxidase isoenzymes on strong anion-exchange monolithic column using pH-salt gradient.
Auteurs : Helena Podgornik [Slovénie] ; Ales PodgornikSource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1570-0232 ] ; 2004.
Descripteurs français
- KwdFr :
- Chromatographie en phase liquide à haute performance (instrumentation), Chromatographie en phase liquide à haute performance (méthodes), Concentration en ions d'hydrogène (MeSH), Focalisation isoélectrique (MeSH), Isoenzymes (isolement et purification), Peroxidases (isolement et purification), Phanerochaete (enzymologie), Résines échangeuses d'anions (MeSH), Sels (composition chimique).
- MESH :
- composition chimique : Sels.
- enzymologie : Phanerochaete.
- isolement et purification : Chromatographie en phase liquide à haute performance, Isoenzymes, Peroxidases.
- méthodes : Chromatographie en phase liquide à haute performance.
- Concentration en ions d'hydrogène, Focalisation isoélectrique, Résines échangeuses d'anions.
English descriptors
- KwdEn :
- Anion Exchange Resins (MeSH), Chromatography, High Pressure Liquid (instrumentation), Chromatography, High Pressure Liquid (methods), Hydrogen-Ion Concentration (MeSH), Isoelectric Focusing (MeSH), Isoenzymes (isolation & purification), Peroxidases (isolation & purification), Phanerochaete (enzymology), Salts (chemistry).
- MESH :
- chemical , chemistry : Salts.
- chemical , isolation & purification : Isoenzymes, Peroxidases.
- chemical : Anion Exchange Resins.
- enzymology : Phanerochaete.
- instrumentation : Chromatography, High Pressure Liquid.
- methods : Chromatography, High Pressure Liquid.
- Hydrogen-Ion Concentration, Isoelectric Focusing.
Abstract
Different chromatographic methods including chromatofocusing are used for separation of manganese peroxidase (MnP) isoforms and their isolation from the fungal growth medium. We tested strong anion exchange methacrylate based monolithic columns as a stationary phase for fast separation of MnP's. Sodium acetate buffers of two different pH values (6 and 4) were used for formation of reproducible pH gradient. The entire cycle, involving analysis and column regeneration, was completed in 3 min. Use of pH gradient showed better MnP isoform separation comparing to the salt gradient, while application of combined pH-salt gradient, resulted in further improvement.
DOI: 10.1016/j.jchromb.2003.10.059
PubMed: 14670754
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Separation of manganese peroxidase isoenzymes on strong anion-exchange monolithic column using pH-salt gradient.</title><author><name sortKey="Podgornik, Helena" sort="Podgornik, Helena" uniqKey="Podgornik H" first="Helena" last="Podgornik">Helena Podgornik</name><affiliation wicri:level="1"><nlm:affiliation>University of Ljubljana, Faculty of Chemistry and Chemical Technology, Askerceva 5, Ljubljana 1000, Slovenia.</nlm:affiliation><country xml:lang="fr">Slovénie</country><wicri:regionArea>University of Ljubljana, Faculty of Chemistry and Chemical Technology, Askerceva 5, Ljubljana 1000</wicri:regionArea><wicri:noRegion>Ljubljana 1000</wicri:noRegion></affiliation></author><author><name sortKey="Podgornik, Ales" sort="Podgornik, Ales" uniqKey="Podgornik A" first="Ales" last="Podgornik">Ales Podgornik</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:14670754</idno><idno type="pmid">14670754</idno><idno type="doi">10.1016/j.jchromb.2003.10.059</idno><idno type="wicri:Area/Main/Corpus">000921</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000921</idno><idno type="wicri:Area/Main/Curation">000921</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000921</idno><idno type="wicri:Area/Main/Exploration">000921</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Separation of manganese peroxidase isoenzymes on strong anion-exchange monolithic column using pH-salt gradient.</title><author><name sortKey="Podgornik, Helena" sort="Podgornik, Helena" uniqKey="Podgornik H" first="Helena" last="Podgornik">Helena Podgornik</name><affiliation wicri:level="1"><nlm:affiliation>University of Ljubljana, Faculty of Chemistry and Chemical Technology, Askerceva 5, Ljubljana 1000, Slovenia.</nlm:affiliation><country xml:lang="fr">Slovénie</country><wicri:regionArea>University of Ljubljana, Faculty of Chemistry and Chemical Technology, Askerceva 5, Ljubljana 1000</wicri:regionArea><wicri:noRegion>Ljubljana 1000</wicri:noRegion></affiliation></author><author><name sortKey="Podgornik, Ales" sort="Podgornik, Ales" uniqKey="Podgornik A" first="Ales" last="Podgornik">Ales Podgornik</name></author></analytic><series><title level="j">Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><idno type="ISSN">1570-0232</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anion Exchange Resins (MeSH)</term><term>Chromatography, High Pressure Liquid (instrumentation)</term><term>Chromatography, High Pressure Liquid (methods)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Isoelectric Focusing (MeSH)</term><term>Isoenzymes (isolation & purification)</term><term>Peroxidases (isolation & purification)</term><term>Phanerochaete (enzymology)</term><term>Salts (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance (instrumentation)</term><term>Chromatographie en phase liquide à haute performance (méthodes)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Focalisation isoélectrique (MeSH)</term><term>Isoenzymes (isolement et purification)</term><term>Peroxidases (isolement et purification)</term><term>Phanerochaete (enzymologie)</term><term>Résines échangeuses d'anions (MeSH)</term><term>Sels (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Salts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Isoenzymes</term><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Anion Exchange Resins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Sels</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Chromatography, High Pressure Liquid</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term><term>Isoenzymes</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hydrogen-Ion Concentration</term><term>Isoelectric Focusing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Concentration en ions d'hydrogène</term><term>Focalisation isoélectrique</term><term>Résines échangeuses d'anions</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Different chromatographic methods including chromatofocusing are used for separation of manganese peroxidase (MnP) isoforms and their isolation from the fungal growth medium. We tested strong anion exchange methacrylate based monolithic columns as a stationary phase for fast separation of MnP's. Sodium acetate buffers of two different pH values (6 and 4) were used for formation of reproducible pH gradient. The entire cycle, involving analysis and column regeneration, was completed in 3 min. Use of pH gradient showed better MnP isoform separation comparing to the salt gradient, while application of combined pH-salt gradient, resulted in further improvement.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14670754</PMID><DateCompleted><Year>2004</Year><Month>04</Month><Day>15</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>22</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1570-0232</ISSN><JournalIssue CitedMedium="Print"><Volume>799</Volume><Issue>2</Issue><PubDate><Year>2004</Year><Month>Jan</Month><Day>25</Day></PubDate></JournalIssue><Title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</Title><ISOAbbreviation>J Chromatogr B Analyt Technol Biomed Life Sci</ISOAbbreviation></Journal><ArticleTitle>Separation of manganese peroxidase isoenzymes on strong anion-exchange monolithic column using pH-salt gradient.</ArticleTitle><Pagination><MedlinePgn>343-7</MedlinePgn></Pagination><Abstract><AbstractText>Different chromatographic methods including chromatofocusing are used for separation of manganese peroxidase (MnP) isoforms and their isolation from the fungal growth medium. We tested strong anion exchange methacrylate based monolithic columns as a stationary phase for fast separation of MnP's. Sodium acetate buffers of two different pH values (6 and 4) were used for formation of reproducible pH gradient. The entire cycle, involving analysis and column regeneration, was completed in 3 min. Use of pH gradient showed better MnP isoform separation comparing to the salt gradient, while application of combined pH-salt gradient, resulted in further improvement.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Podgornik</LastName><ForeName>Helena</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>University of Ljubljana, Faculty of Chemistry and Chemical Technology, Askerceva 5, Ljubljana 1000, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Podgornik</LastName><ForeName>Ales</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Chromatogr B Analyt Technol Biomed Life Sci</MedlineTA><NlmUniqueID>101139554</NlmUniqueID><ISSNLinking>1570-0232</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000837">Anion Exchange Resins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007527">Isoenzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012492">Salts</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000837" MajorTopicYN="Y">Anion Exchange Resins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="Y">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007525" MajorTopicYN="N">Isoelectric Focusing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007527" MajorTopicYN="N">Isoenzymes</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012492" MajorTopicYN="N">Salts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>12</Month><Day>13</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>4</Month><Day>16</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>12</Month><Day>13</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14670754</ArticleId><ArticleId IdType="pii">S1570023203009048</ArticleId><ArticleId IdType="doi">10.1016/j.jchromb.2003.10.059</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Slovénie</li></country></list><tree><noCountry><name sortKey="Podgornik, Ales" sort="Podgornik, Ales" uniqKey="Podgornik A" first="Ales" last="Podgornik">Ales Podgornik</name></noCountry><country name="Slovénie"><noRegion><name sortKey="Podgornik, Helena" sort="Podgornik, Helena" uniqKey="Podgornik H" first="Helena" last="Podgornik">Helena Podgornik</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000864 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000864 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:14670754
|texte= Separation of manganese peroxidase isoenzymes on strong anion-exchange monolithic column using pH-salt gradient.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:14670754" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |